Aspirate 8 channels from different plates, one command

rickwierenga · January 30, 2025, 1:01am

STARChatterboxBackend?

sorry it’s confusing, but i have a concept of a plan that I will soon implement to fix it all

mackenzie · January 30, 2025, 1:13am

I don’t seem to have STARChatterboxBackend so I’m using LiquidHandlerChatterboxBackend

rickwierenga · January 30, 2025, 2:40am

the same as STAR, except it prints out all firmware commands instead of sending them to the machine

github.com

PyLabRobot/pylabrobot/blob/2b3a14f29c620e9f5eb2b2beb41d950bbcd90bdc/pylabrobot/liquid_handling/backends/hamilton/STAR_chatterbox.py#L7


      
          from typing import Optional
          
          from pylabrobot.liquid_handling.backends import LiquidHandlerBackend
          from pylabrobot.liquid_handling.backends.hamilton.STAR import STAR
          
          
          class STARChatterboxBackend(STAR):
            """Chatterbox backend for "STAR" """
          
            def __init__(self, num_channels: int = 8):
              """Initialize a chatter box backend."""
              super().__init__()
              self._num_channels = num_channels
              self._iswap_parked = True
          
            async def setup(self, skip_autoload=False, skip_iswap=False, skip_core96_head=False) -> None:
              await LiquidHandlerBackend.setup(self)

mackenzie · January 30, 2025, 4:28am

ValueError: Minimum distance between two y positions is <9mm: 4982, 4982 (channel 0 and 1)

this error arises after

C0TRid0045xp13400 13400 13400 13400 13400 13400 00000&yp3973 3629 3284 2940 2595 2251 0000&tm1 1 1 1 1 1 0&tp1970tz1870th2450te2450ti0
C0TPid0046xp03249 03249 03249 03249 03249 03249 00000&yp1278 1188 1098 1008 0918 0828 0000&tm1 1 1 1 1 1 0&tt01tp2244tz2164th2450td0

this comes after a dispense command that works properly with 6 channels in 1 well of the 12-channel trough

rickwierenga · January 30, 2025, 4:50pm

if the error arises, no firmware command is generated. The above tip pickup and drop firmware commands look normal.

I have no idea what your issue is. “On this note, input ['A12', 'A11', 'A10', 'A9', 'A8', 'A7'] as the “sources” for aspirate and got the error that channel 0 and channel 1 were too close together because the y position was the same.” which plate and in which rotation?

mackenzie · January 30, 2025, 6:45pm

The nest_12_troughplate_15000uL_Vb in the standard orientation