HamiltonStar 16 Channel Aspiration Error

Hello! Ive tested the Pyalbrobot for the first time last week, first of all what an awesome tool to be able to use! Thanks alot for developing this!

I stumbled upon a problem with the aspirate function. I have a Hamilton Star with 16 individual channels to aspirate from. I checked each individual channel with the “use_channels” command [0-15]. I can pick up tips without a problem for each channel, however the aspiration for last two cahnnels [14,15] gives the error:

pylabrobot.liquid_handling.backends.hamilton.STAR_backend.STARFirmwareError: {‘Master’: CommandSyntaxError(‘Parameter out of range’)}, C0ASid0010er01/32

The third last channel [13] does not seem to be working with the aspirate function either, however this does not give a direct error but a timeout response.

I will post the script below i used to test the different channels. perhaps i missed some argument or need a special configuration for the 16 channel setup?

==== Code ====

import asyncio
from pylabrobot.resources import (
    TIP_CAR_480_A00,
    PLT_CAR_L5AC_A00,
    Cor_96_wellplate_360ul_Fb,
    Tube_CAR_32_A00,
    HTF,
    Tube
)

from pylabrobot.resources.coordinate import Coordinate
from pylabrobot.liquid_handling import LiquidHandler
from pylabrobot.liquid_handling.backends import STARBackend
from pylabrobot.resources.hamilton import HamiltonSTARDeck

async def main():
    deck = HamiltonSTARDeck(num_rails=54, size_x=1215,size_y=465,size_z=195)
    lh = LiquidHandler(backend=STARBackend(), deck=deck)
    await lh.setup()

    tip_car = TIP_CAR_480_A00(name="tip carrier")
    tip_car[0] = HTF(name="tips_01")
    lh.deck.assign_child_resource(tip_car, rails=42)  # rail 42 exists on HamiltonSTARDeck

    plt_car = PLT_CAR_L5AC_A00(name="plate carrier")
    plt_car[0] = Cor_96_wellplate_360ul_Fb(name="plate_01")
    lh.deck.assign_child_resource(plt_car, rails=7)

    tube_car = Tube_CAR_32_A00(name="serum tube carrier")

    for i in range(32):
        tube_car[i] = Tube(
            name=f"tube_{i + 1:02}",  # zero-padded: tube_01, tube_02, ..., tube_32
            size_x=13,
            size_y=13,
            size_z=90,
            max_volume=2000,
            material_z_thickness=1.5
        )

    lh.deck.assign_child_resource(tube_car, rails=35)

    print(lh.summary())

    tiprack = lh.deck.get_resource("tips_01")
    await lh.pick_up_tips(tiprack["G1","H1"],use_channels=[6,7])
    print("tip location: ",tiprack.get_absolute_location())

    tube_1 = lh.deck.get_resource("tube_01")
    tube_2 = lh.deck.get_resource("tube_02")

    await lh.aspirate(
        resources=[tube_2, tube_1],
        use_channels=[6, 7],
        vols=[20, 20],
        lld_mode=[STARBackend.LLDMode.GAMMA, STARBackend.LLDMode.GAMMA],
        flow_rates=[25, 25],
        surface_following_distance=[4, 4]
    )

    plate = lh.deck.get_resource("plate_01")
    await lh.dispense(
        resources=plate["G1","H1"],
        vols=[20, 20],
        use_channels=[6, 7],
        offsets=[Coordinate(0, 0, 5), Coordinate(0, 0, 5)]
    )


    await lh.return_tips()
    await lh.stop()
    print("Script completed successfully")

if __name__ == "__main__":
    asyncio.run(main())

welcome to the forum!

what is most likely the case is that the channels can’t physically reach the front of the robot. with 16 channels, there isn’t full random access due to the other channels being in the way

can you verify channels 14 and 15 work on different tubes, somewhere in the middle of the robot?

that is weird. could you share a full firmware log?

1 Like

Hi Rick, thanks for your reply!

The strange thing is the positions are quite reachable, the tip channels 14,15 are the outermost tips, tip channels further in can even reach the outermost aspiration / dispense positions.

I also tested the aspiration and dispense with the last three channels that have been giving me trouble 13,14,15 in the Venus software where they work fine.

Is each pipetting channel restricted by the software somewhere that is incorrectly configured in this case?

Let me get back to you with the log on the channel 13 that gave a response time error when I’m back in the lab tomorrow.

1 Like

that is interesting. could you also send me the trc file? i am curious about how their firmware command differs from ours (dm is fine if you need privacy)

to confirm: you are using the same aspiration parameters for all channels right? like exact same call, except use_channels is different right?

1 Like

Hi Rick, thanks again for looking into this!

Im not sure how to run the full firmware log correctly. i initally got an attribution error when trying to run it as described in the link you provided. I managed to locate it under pylabrobot.pylabrobot.io. The start and stop_capture() did work, however only the version was writen. i think only lines 34-39 in the “capture.py” was executed. this was the output to the JSON file:

{
“version”: “0.1.6”,
“commands”:
}

I have made a script to try and debug this aspiration. All variables remain the same, i only change the integer in the “use_channels” argument. ill provide the script and the print logs thereoff below. To summeriye, channel 0-12 work fine, printlogs look similar, channel 13 gives a timeout response error, channel 14,15 gives an parameter our of range error.

=== script ===

import asyncio
import pylabrobot.pylabrobot.io as plr
from pylabrobot.resources import (
    TIP_CAR_480_A00,
    PLT_CAR_L5AC_A00,
    Cor_96_wellplate_360ul_Fb,
    HTF,
)
from pylabrobot.liquid_handling import LiquidHandler
from pylabrobot.liquid_handling.backends import STARBackend
from pylabrobot.resources.hamilton import HamiltonSTARDeck

async def main():
    deck = HamiltonSTARDeck(num_rails=54, size_x=1215, size_y=465, size_z=195)
    backend = STARBackend()
    lh = LiquidHandler(backend=backend, deck=deck)
    await lh.setup()

    # print debug info and commands sent
    orig_aspirate = backend.aspirate
    async def debug_aspirate(*args, **kwargs):
        ops = kwargs["ops"]
        use_channels = kwargs["use_channels"]
        x_positions, y_positions, channels_involved = backend._ops_to_fw_positions(ops, use_channels)

        print("\n DEBUG ASPIRATE")
        print(f"  use_channels:      {use_channels}")
        print(f"  tip_pattern:       {channels_involved}")
        print(f"  x_positions (mm):  {x_positions}")
        print(f"  y_positions (mm):  {y_positions}")
        print(f"  volumes (tenths):  {[round(op.volume * 10) for op in ops]}")

        # Try catching exceptions
        try:
            return await orig_aspirate(*args, **kwargs)
        except Exception as e:
            print(f" ERROR during aspirate with channels {use_channels}: {e}")
            raise

    backend.aspirate = debug_aspirate

    # Setup carriers and resources
    tip_car = TIP_CAR_480_A00("tip_car")
    tip_car[0] = HTF("tips_01")
    lh.deck.assign_child_resource(tip_car, rails=42)

    plt_car = PLT_CAR_L5AC_A00("plate_car")
    plt_car[0] = Cor_96_wellplate_360ul_Fb("plate_01")
    lh.deck.assign_child_resource(plt_car, rails=7)

    print(lh.summary())
    test_channels = [0]
    # Test all channels 0 through 15 individually to isolate failure
    for test_channel in test_channels:
        print(f"\n=== Testing channel {test_channel} ===")
        tiprack = lh.deck.get_resource("tips_01")
        try:
            validation_file = "16_channel_validation.json"
            plr.start_capture(validation_file)
            await lh.pick_up_tips(tiprack["A1"], use_channels=[test_channel])

            plate = lh.deck.get_resource("plate_01")
            await lh.aspirate(
                plate["A4"],
                use_channels=[test_channel],
                vols=[10]
            )

            await lh.dispense(
                plate["A5"],
                vols=[10],
                use_channels=[test_channel],
            )

            await lh.return_tips()

            print(f" Channel {test_channel} aspirate/dispense succeeded")
            plr.stop_capture()

        except Exception as e:
            print(f" Channel {test_channel} failed with error:\n{e}")

    await lh.stop()
    print("\n Script completed.")


if __name__ == "__main__":
    asyncio.run(main())

=== printlog (channel 0) ===
Rail Resource Type Coordinates (mm)

(-6) ├── trash_core96 Trash (-58.200, 106.000, 229.000)

(7) ├── plate_car PlateCarrier (235.000, 063.000, 100.000)
│ ├── plate_01 Plate (239.000, 071.500, 183.120)
│ ├──
│ ├──
│ ├──
│ ├──

(25) ├── trash Trash (655.000, 190.600, 137.100)

(42) ├── tip_car TipCarrier (1022.500, 063.000, 100.000)
│ ├── tips_01 TipRack (1028.700, 073.000, 214.950)
│ ├──
│ ├──
│ ├──
│ ├──

(53) ├── waste_block Resource (1270.000, 115.000, 100.000)
│ ├── teaching_tip_rack TipRack (1275.900, 461.100, 100.000)

None

=== Testing channel 1 ===

DEBUG ASPIRATE
use_channels: [1]
tip_pattern: [False, True, False]
x_positions (mm): [0, 2803, 0]
y_positions (mm): [0, 1457, 0]
volumes (tenths): [100]
Channel 1 aspirate/dispense succeeded
Validation file written to 16_channel_validation.json

Script completed.

=== printlog (channel 13) ===

Rail Resource Type Coordinates (mm)

(-6) ├── trash_core96 Trash (-58.200, 106.000, 229.000)

(7) ├── plate_car PlateCarrier (235.000, 063.000, 100.000)
│ ├── plate_01 Plate (239.000, 071.500, 183.120)
│ ├──
│ ├──
│ ├──
│ ├──

(25) ├── trash Trash (655.000, 190.600, 137.100)

(42) ├── tip_car TipCarrier (1022.500, 063.000, 100.000)
│ ├── tips_01 TipRack (1028.700, 073.000, 214.950)
│ ├──
│ ├──
│ ├──
│ ├──

(53) ├── waste_block Resource (1270.000, 115.000, 100.000)
│ ├── teaching_tip_rack TipRack (1275.900, 461.100, 100.000)

None

=== Testing channel 13 ===

DEBUG ASPIRATE
use_channels: [13]
tip_pattern: [False, False, False, False, False, False, False, False, False, False, False, False, False, True, False]
x_positions (mm): [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2803, 0]
y_positions (mm): [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1457, 0]
volumes (tenths): [100]
Timeout while waiting for response to command C0ASid0009at0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&tm0 0 0 0 0 0 0 0 0 0 0 0 0 1 0&xp00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 02803 00000&yp0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1457 0000&th2450te2450lp2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000&ch000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&zl1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866&po0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100&zu0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032&zr06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180&zx1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866&ip0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&it0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&fp0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&av00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127&as2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500&ta000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ba0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&oa000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&lm0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&ll1 1 1 1 1 1 1 1 1 1 1 1 1 1 1&lv1 1 1 1 1 1 1 1 1 1 1 1 1 1 1&zo000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ld00 00 00 00 00 00 00 00 00 00 00 00 00 00 00&de0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020&wt10 10 10 10 10 10 10 10 10 10 10 10 10 10 10&mv00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000&mc00 00 00 00 00 00 00 00 00 00 00 00 00 00 00&mp000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ms1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200&mh0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&gi000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&gj0gk0lk0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&ik0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&sd0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500&se0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500&sz0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300&io0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&il00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000&in0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&.
ERROR during aspirate with channels [13]: Timeout while waiting for response to command C0ASid0009at0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&tm0 0 0 0 0 0 0 0 0 0 0 0 0 1 0&xp00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 02803 00000&yp0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1457 0000&th2450te2450lp2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000&ch000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&zl1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866&po0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100&zu0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032&zr06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180&zx1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866&ip0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&it0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&fp0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&av00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127&as2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500&ta000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ba0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&oa000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&lm0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&ll1 1 1 1 1 1 1 1 1 1 1 1 1 1 1&lv1 1 1 1 1 1 1 1 1 1 1 1 1 1 1&zo000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ld00 00 00 00 00 00 00 00 00 00 00 00 00 00 00&de0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020&wt10 10 10 10 10 10 10 10 10 10 10 10 10 10 10&mv00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000&mc00 00 00 00 00 00 00 00 00 00 00 00 00 00 00&mp000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ms1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200&mh0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&gi000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&gj0gk0lk0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&ik0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&sd0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500&se0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500&sz0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300&io0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&il00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000&in0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&.
Channel 13 failed with error:
Timeout while waiting for response to command C0ASid0009at0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&tm0 0 0 0 0 0 0 0 0 0 0 0 0 1 0&xp00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 02803 00000&yp0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1457 0000&th2450te2450lp2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000&ch000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&zl1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866&po0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100 0100&zu0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032 0032&zr06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180 06180&zx1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866 1866&ip0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&it0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&fp0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&av00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127 00127&as2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500 2500&ta000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ba0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&oa000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&lm0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&ll1 1 1 1 1 1 1 1 1 1 1 1 1 1 1&lv1 1 1 1 1 1 1 1 1 1 1 1 1 1 1&zo000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ld00 00 00 00 00 00 00 00 00 00 00 00 00 00 00&de0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020 0020&wt10 10 10 10 10 10 10 10 10 10 10 10 10 10 10&mv00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000&mc00 00 00 00 00 00 00 00 00 00 00 00 00 00 00&mp000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&ms1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200&mh0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&gi000 000 000 000 000 000 000 000 000 000 000 000 000 000 000&gj0gk0lk0 0 0 0 0 0 0 0 0 0 0 0 0 0 0&ik0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&sd0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500&se0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500 0500&sz0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300 0300&io0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&il00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000&in0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000&.

Script completed.

=== printlog (channel 14) ===
Rail Resource Type Coordinates (mm)

(-6) ├── trash_core96 Trash (-58.200, 106.000, 229.000)

(7) ├── plate_car PlateCarrier (235.000, 063.000, 100.000)
│ ├── plate_01 Plate (239.000, 071.500, 183.120)
│ ├──
│ ├──
│ ├──
│ ├──

(25) ├── trash Trash (655.000, 190.600, 137.100)

(42) ├── tip_car TipCarrier (1022.500, 063.000, 100.000)
│ ├── tips_01 TipRack (1028.700, 073.000, 214.950)
│ ├──
│ ├──
│ ├──
│ ├──

(53) ├── waste_block Resource (1270.000, 115.000, 100.000)
│ ├── teaching_tip_rack TipRack (1275.900, 461.100, 100.000)

None

=== Testing channel 14 ===

DEBUG ASPIRATE
use_channels: [14]
tip_pattern: [False, False, False, False, False, False, False, False, False, False, False, False, False, False, True, False]
x_positions (mm): [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2803, 0]
y_positions (mm): [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1457, 0]
volumes (tenths): [100]
ERROR during aspirate with channels [14]: {‘Master’: CommandSyntaxError(‘Parameter out of range’)}, C0ASid0010er01/32
Channel 14 failed with error:
{‘Master’: CommandSyntaxError(‘Parameter out of range’)}, C0ASid0010er01/32

Script completed.

I apologize for the poor formatting …

I was able to run the cahnnels 13,14,15 in hamilton venus without an issue. Im not sure how to extract the trc file so i took a screnshot:

Hi @KarlCahill,
The .trc file is autogenerated by VENUS and stored in your folders under HAMILTON\LogFiles.

The .trc file usually just has the date as its name, i.e. if you run multiple Hamilton “method” files on the same day, all their firmware logs will be stored in the same .trc file.

Thank you!

Here is the trace file in text:

2025-07-08 10:45:39> SYSTEM : Analyze method - start; Method file C:\Program Files (x86)\Hamilton\Methods\Atanis_test_2.hsl
2025-07-08 10:45:39> Microlab® STAR : Communication - progress; Connection to instrument is created.
2025-07-08 10:45:41> SYSTEM : Analyze method - complete;
2025-07-08 10:45:46> SYSTEM : Start method - start;
2025-07-08 10:45:46> SYSTEM : Start method - progress; User name: Hamilton
2025-07-08 10:45:46> SYSTEM : Start method - progress; Phoenix software version: 4.5.0.7977
2025-07-08 10:45:47> SYSTEM : Start method - progress; Database version: Standard
2025-07-08 10:45:47> SYSTEM : Start method - progress; Sample tracking: Off
2025-07-08 10:45:47> SYSTEM : Start method - progress; Vector Database: On
2025-07-08 10:45:47> SYSTEM : Start method - progress; Vector Database connection: Database ‘HamiltonVectorDB’ on Microsoft SQL Server ‘LOCALHOST\HAMILTON’ with user ‘Hamilton’
2025-07-08 10:45:47> Microlab® STAR : Start method command - start;
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Microlab® STAR software version: 4.5.0.5217
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Serial number of Instrument: 212I
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of main module: 7.5S 19 2020_09_04 (GRU C0)
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of 1000µl channels: 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31, 4.0S f 2020-07-31
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of 5ml channels: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of labware handling channels: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of X drive: 1.4S 2012-04-25
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of auto load: 3.4S e 2016-05-02
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of iSWAP: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of washer 1: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of washer 2: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of temperature-controlled carrier 1: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of temperature-controlled carrier 2: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of CO-RE 96 Head: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of pump station 1: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of pump station 2: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of pump station 3: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of nano dispenser: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of nano dispenser pressure unit: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of CO-RE 384 Head: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of tube gripper: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of camera channel: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of gel card gripper: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of punch card gripper: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of puncher module: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 1 module 1: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 1 module 2: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 2 module 1: not installed
2025-07-08 10:45:47> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 2 module 2: not installed
2025-07-08 10:45:51> Microlab® STAR : Start method command - complete;
2025-07-08 10:45:51> SYSTEM : Start method - complete;
2025-07-08 10:45:51> SYSTEM : Execute method - start; Method file C:\Program Files (x86)\Hamilton\Methods\Atanis_test_2.hsl
2025-07-08 10:45:51> Microlab® STAR : 1000ul Channel Aspirate - start;
2025-07-08 10:45:51> Microlab® STAR : Initialize (Single Step) - start;
2025-07-08 10:45:51> Microlab® STAR : Initialize (Single Step) - complete; > channel 1: Waste, > channel 2: Waste, > channel 3: Waste, > channel 4: Waste, > channel 5: Waste, > channel 6: Waste, > channel 7: Waste, > channel 8: Waste, > channel 9: Waste, > channel 10: Waste, > channel 11: Waste, > channel 12: Waste, > channel 13: Waste, > channel 14: Waste, > channel 15: Waste, > channel 16: Waste,
2025-07-08 10:45:51> Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2025-07-08 10:45:58> Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - progress; Error handling waiting for manual recovery!
2025-07-08 10:46:12> Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - error; > channel 16: HTF_L_0002, 1, E:P16:08/75:Repeat
2025-07-08 10:46:12> Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - progress; Error manually recovered by user.
2025-07-08 10:46:17> Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;
2025-07-08 10:46:17> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2025-07-08 10:46:27> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 16: Fal_96_Rd_0001, A4, 10 uL
2025-07-08 10:46:27> Microlab® STAR : 1000ul Channel Aspirate - complete;
2025-07-08 10:46:27> Microlab® STAR : 1000ul Channel Dispense - start;
2025-07-08 10:46:27> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2025-07-08 10:46:31> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 16: Fal_96_Rd_0001, A5, 10 uL
2025-07-08 10:46:31> Microlab® STAR : 1000ul Channel Dispense - complete;
2025-07-08 10:46:31> SYSTEM : Execute method - complete;
2025-07-08 10:46:31> SYSTEM : End method - start;
2025-07-08 10:46:31> Microlab® STAR : End method command - start;
2025-07-08 10:46:32> Microlab® STAR : Clean up instrument - progress; Move up 1000ul channels
2025-07-08 10:46:32> Microlab® STAR : Clean up instrument - progress; Move down auto load Y drive
2025-07-08 10:46:42> Microlab® STAR : - progress; User action: Message box ‘Clean up instrument’ message ‘Please check the traverse height! Is there any indication for a collision when moving horizontally?’ was closed with button ‘No’.
2025-07-08 10:46:42> Microlab® STAR : Clean up instrument - progress; Eject picked up needle from channel
2025-07-08 10:46:42> Microlab® STAR : Clean up instrument - progress; Eject picked up tip from channel
2025-07-08 10:46:50> Microlab® STAR : Clean up instrument - progress; Move up 1000ul channels
2025-07-08 10:46:50> Microlab® STAR : Clean up instrument - progress; Move down auto load Y drive
2025-07-08 10:46:51> Microlab® STAR : Clean up instrument - progress; Spreading channels
2025-07-08 10:46:53> Microlab® STAR : Clean up instrument - progress; Switch off loading lights
2025-07-08 10:46:54> Microlab® STAR : End method command - complete;
2025-07-08 10:46:54> SYSTEM : End method - complete;
2025-07-08 10:52:48> Microlab® STAR : Communication - progress; Connection to instrument is interrupted.
2025-07-08 13:45:22> SYSTEM : File checksum - written; $$author=Hamilton$$valid=0$$time=2025-07-08 13:45$$checksum=175880d8$$length=137$$

I think we’re almost there:

Is this the file named HxUsbComm20250708.trc?

(assuming you executed this script today)

1 Like

Ah no the previous trc file was named “Atanis_test_2_ddd060ab33d5411594f7984163f1fe0d_Trace.trc”

Btw i am not permitted to upload .txt files, the file is a bit too extensive to paste in one go, ill write it in two replies:

here is the file you mention for HxUsbComm20250708.trc(1/2):
< 10:42:41.241 8AF#8000#00: C0RQid0101

10:42:41.257 8AF#8000#00: C0RQid0101rq0000
< 10:42:41.260 8AF#8000#00: C0QBid0102
10:42:41.276 8AF#8000#00: C0QBid0102er00/00qb1
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10:42:41.296 8AF#8000#00: C0RIid0103er00/00si2022-01-12sn212I
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10:42:41.324 8AF#8000#00: C0QMid0104er00/00ka000000xt54xa54xw13400xl01xr00xm03500xx11400ys090xu3540xv3700yu0060kl360kc0yx0060ke00000100xn00xo00ym6065kr0km360
< 10:42:41.324 8AF#8000#00: C0RMid0105
10:42:41.425 8AF#8000#00: C0RMid0105er00/00kb0Dkp16 C00000 X00000 P10000 P20000 P30000 P40000 P50000 P60000 P70000 P80000 P90000 PA0000 PB0000 PC0000 PD0000 PE0000 PF0000 PG0000 I00000
< 10:42:41.426 8AF#8000#00: C0RFid0106
10:42:41.437 8AF#8000#00: C0RFid0106er00/00rf7.5S 19 2020_09_04 (GRU C0)
< 10:42:41.437 8AF#8000#00: P1RFid0107
10:42:41.459 8AF#8000#00: P1RFid0107rf4.0S f 2020-07-31
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10:42:41.471 8AF#8000#00: P1RJid0108jd2025-05-12js1
< 10:42:41.471 8AF#8000#00: P2RFid0109
10:42:41.482 8AF#8000#00: P2RFid0109rf4.0S f 2020-07-31
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10:42:41.497 8AF#8000#00: P2RJid0110jd2025-05-12js1
< 10:42:41.497 8AF#8000#00: P3RFid0111
10:42:41.510 8AF#8000#00: P3RFid0111rf4.0S f 2020-07-31
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10:42:41.523 8AF#8000#00: P3RJid0112jd2025-05-12js1
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10:42:41.532 8AF#8000#00: P4RFid0113rf4.0S f 2020-07-31
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10:42:41.549 8AF#8000#00: P4RJid0114jd2025-05-12js1
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10:42:41.549 8AF#8000#00: P5RFid0115rf4.0S f 2020-07-31
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10:42:41.565 8AF#8000#00: P5RJid0116jd2025-05-12js1
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10:42:41.581 8AF#8000#00: P6RFid0117rf4.0S f 2020-07-31
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10:42:41.599 8AF#8000#00: P6RJid0118jd2025-05-12js1
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10:42:41.610 8AF#8000#00: P7RFid0119rf4.0S f 2020-07-31
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10:42:41.622 8AF#8000#00: P7RJid0120jd2025-05-12js1
< 10:42:41.623 8AF#8000#00: P8RFid0121
10:42:41.633 8AF#8000#00: P8RFid0121rf4.0S f 2020-07-31
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10:42:41.645 8AF#8000#00: P8RJid0122jd2025-05-12js1
< 10:42:41.647 8AF#8000#00: P9RFid0123
10:42:41.659 8AF#8000#00: P9RFid0123rf4.0S f 2020-07-31
< 10:42:41.659 8AF#8000#00: P9RJid0124
10:42:41.671 8AF#8000#00: P9RJid0124jd2025-05-12js1
< 10:42:41.671 8AF#8000#00: PARFid0125
10:42:41.683 8AF#8000#00: PARFid0125rf4.0S f 2020-07-31
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10:42:41.696 8AF#8000#00: PARJid0126jd2025-05-12js1
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10:42:41.708 8AF#8000#00: PBRFid0127rf4.0S f 2020-07-31
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10:42:41.721 8AF#8000#00: PBRJid0128jd2025-05-12js1
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10:42:41.726 8AF#8000#00: PCRFid0129rf4.0S f 2020-07-31
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10:42:41.747 8AF#8000#00: PCRJid0130jd2025-05-12js1
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10:42:41.759 8AF#8000#00: PDRFid0131rf4.0S f 2020-07-31
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10:42:41.773 8AF#8000#00: PDRJid0132jd2025-05-12js1
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10:42:41.785 8AF#8000#00: PERFid0133rf4.0S f 2020-07-31
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10:42:41.796 8AF#8000#00: PERJid0134jd2025-05-12js1
< 10:42:41.796 8AF#8000#00: PFRFid0135
10:42:41.813 8AF#8000#00: PFRFid0135rf4.0S f 2020-07-31
< 10:42:41.813 8AF#8000#00: PFRJid0136
10:42:41.826 8AF#8000#00: PFRJid0136jd2025-05-12js1
< 10:42:41.826 8AF#8000#00: PGRFid0137
10:42:41.833 8AF#8000#00: PGRFid0137rf4.0S f 2020-07-31
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10:42:41.851 8AF#8000#00: PGRJid0138jd2025-05-12js1
< 10:42:41.851 8AF#8000#00: C0QBid0139
10:42:41.854 8AF#8000#00: C0QBid0139er00/00qb1
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10:42:41.870 8AF#8000#00: X0RFid0140rf1.4S 2012-04-25
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10:42:41.890 8AF#8000#00: X0RJid0141jd2025-05-12js1
< 10:42:41.891 8AF#8000#00: I0RFid0142
10:42:41.893 8AF#8000#00: I0RFid0142rf3.4S e 2016-05-02
< 10:42:41.902 8AF#8000#00: I0RJid0143
10:42:41.902 8AF#8000#00: I0RJid0143jd2022-01-12js1
< 10:42:41.918 8AF#8000#00: C0RMid0144
10:42:42.016 8AF#8000#00: C0RMid0144er00/00kb0Dkp16 C00000 X00000 P10000 P20000 P30000 P40000 P50000 P60000 P70000 P80000 P90000 PA0000 PB0000 PC0000 PD0000 PE0000 PF0000 PG0000 I00000
< 10:42:42.029 8AF#8000#00: C0QMid0145
10:42:42.075 8AF#8000#00: C0QMid0145er00/00ka000000xt54xa54xw13400xl01xr00xm03500xx11400ys090xu3540xv3700yu0060kl360kc0yx0060ke00000100xn00xo00ym6065kr0km360
< 10:42:42.078 8AF#8000#00: C0SRid0146
10:42:42.094 8AF#8000#00: C0SRid0146er00/00sr055
< 10:42:42.109 8AF#8000#00: C0RUid0147
10:42:42.148 8AF#8000#00: C0RUid0147er00/00ru00950 13400 30000 30000
< 10:42:45.121 8AF#8000#00: C0RVid0148vo00
10:42:45.168 8AF#8000#00: C0RVid0148er00/00vd2025-06-30 10:25vs0
< 10:42:45.168 8AF#8000#00: C0RVid0149vo01
10:42:45.234 8AF#8000#00: C0RVid0149er00/00vd2025-05-12 15:34vs1
< 10:42:45.247 8AF#8000#00: C0RVid0150vo05
10:42:45.297 8AF#8000#00: C0RVid0150er00/00vd2023-03-11 23:59vs1
< 10:42:45.297 8AF#8000#00: C0RSid0151
10:42:45.327 8AF#8000#00: C0RSid0151er00/00ts2025-06-30 10:30
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10:42:45.343 8AF#8000#00: C0RIid0152er00/00si2022-01-12sn212I
< 10:42:45.343 8AF#8000#00: C0RMid0153
10:42:45.437 8AF#8000#00: C0RMid0153er00/00kb0Dkp16 C00000 X00000 P10000 P20000 P30000 P40000 P50000 P60000 P70000 P80000 P90000 PA0000 PB0000 PC0000 PD0000 PE0000 PF0000 PG0000 I00000
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10:42:45.841 8AF#8000#00: PCVWid0181vw0 0
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10:42:45.890 8AF#8000#00: PFVWid0184vw0 0
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10:45:40.436 8AF#8000#00: PERFid0133rf4.0S f 2020-07-31
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10:45:40.451 8AF#8000#00: PERJid0134jd2025-05-12js1
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10:45:40.464 8AF#8000#00: PFRFid0135rf4.0S f 2020-07-31
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10:45:40.488 8AF#8000#00: PGRFid0137rf4.0S f 2020-07-31
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10:45:40.504 8AF#8000#00: PGRJid0138jd2025-05-12js1
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10:45:40.569 8AF#8000#00: I0RJid0143jd2022-01-12js1
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! 10:52:48.735 8AF#8000#00: USB Info: DEVICE_REMOVE_COMPLETE: \?\USB#VID_08AF&PID_8000#00#{bd0dab87-838b-44c5-a114-1cba5be0e45f}
! 10:52:48.822 8AF#8000#00: USB Info: ABORT_PIPE
! 10:52:48.823 8AF#8000#00: While reading from USBIO Driver: An error occurred while running Vector. The error description is: Error code 0xE0000011: HC Error: XACT error. (0x33 - 0x1 - 0x0)

fixed

thank you for sharing all that information.

  • the venus script you are running appears to be different from the PLR script:
    • the venus log is using 15 channels, the PLR tracebacks are 13 and 14 channels
    • in the 13 channel PLR firmware command (timeout): i see the y-parameter is 146.0mm (for the 13 channel one, we don’t know for 14) whereas in venus it is 405mm. (this should not make a difference since the front channels should be to access the front of the robot, but something to keep in mind)
  • to clearly see where things are going wrong, could you please share the validation.json for channels 12 (works), 13 (timeout), 14 (error), 16 (error)?

Hi Rick, thanks again!

Sorry for the confusion with running the different channels. I ran the aspiration again with the venus for channels 12,13,14,15 (0 based indexed) numbered 13,14,15,16 in venus.

I am permitted to upload .txt files now but get the error:
“Sorry, new users can not upload attachments.”

I couldent quite get the capture function to work to write to the JSON file. could you confirm that i am using it correctly in the script posted previously (post 5)

1 Like

@KarlCahill, in the absence of the validation.json, could you please share this run’s .tcr file (containing the firmware log)?

Here is some more info from the documents Hamilton made freely available via the labautomation forum:

and here is an infographic I just generated that shows details of what the above is referring to with specific y-coordinates accessible by different channel configurations

Note: this shows “random access space”. It does not show what the range of each individual channel is!
I will also generate a table that shows the y-range for each specific channel in the next couple of days which should help us with this (and we can then add to the PLR docs :slight_smile: )

1 Like

ah, so there were two problems :slight_smile:

  • txt and json were not allowed file types for upload
  • you were still a new user (i upgraded your trust level)

Thanks alot guys!

Here is the .trc file from the venus logfile from when i run the channels 12,13,14,15 yesterday. All ran sucsesfully with the venus software.

HxUsbComm20250709.txt (113.4 KB)

Here is the layout from the hamilton:

I tested the same channels with pylabrobot, and the issues for the last three cahnnels persisted, parameter out of range for 14,15 and timeout error with channel 13. channel 12 works fine.

The channels that gives these errors when aspirating are able to pick up tips fine in the outer most and second outermost positions “TIP_CAR_480_A00[0]” and “TIP_CAR_480_A00” from all positions A-H. This indicates that the random acsess space for the y dimension is fine for for these channels.

I still havent figured out how to get the “capture” to work to the JSON file. Is there an alternative way we can print or extract the commands sent similar to the .trc file for pylabrobot?

2 Likes

@KarlCahill, yes: PLR automatically generates a log file too. You just have to choose what you want to capture by choosing the the log level.

Add the following at the beginning of your script:

from pylabrobot.io.validation_utils import LOG_LEVEL_IO

logger = logging.getLogger("pylabrobot")
logger.setLevel(LOG_LEVEL_IO) # <-- choose a level

This was (relatively) recently changed, please let us know whether you can see the PLR-generated log file in the same directory as your script.

1 Like

Fantastic, thanks for the update, i managed to use the capture command now!

The Capture worked correctly for channel 12 which can aspirate without an error:
channel_12_test.txt (6.4 KB)

For the failed aspiration test it could not write into the JSON with the capture function. I instead managed to find the commands sent through the “pylabrobot_io_log.json”. I organized them by into files of the respective tests, also including the cahannel 12 again that worked correctly:

channel_12_test_from_log.txt (10.3 KB)
channel_13_test_from_log.txt (9.8 KB)
channel_14_test_from_log.txt (7.8 KB)
channel_15_test_from_log.txt (7.3 KB)

I hope this gives the insight you need, let me know if i should run more tests. and thanks again!

2 Likes

Hey guys,
Any idea how to trouble shoot further or ideas to solve the problem?

Best regards
Karl